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Re: Prion research and mad cow disease



Dear Judith,
you asked the 5 c Christmas questions. I think ~30,000+++ of the world's foremost biologists, genticists, pharmac(olog)ists, chemists,
medics, physiologists and even normal smart people would like to have those answers, many thousands are even working on it - some even very well paid.
 
A mad cow can startle many smart people.
 
Why am I joking about such tragic topics? First: I am joking about everything, it keeps the mind creatively working. My wife always warns about keeping selective in it, because many people cannot cope with flippancy when they want to be pretentious. Here I have a non-flippant reason for some humor:
 
The prions (you got that absolutely right with all its connotations) are the latest marvels what science could "think" about. Nothing essential, beyond a superficial identification. I think the most weird point is what you wrote: that the immune system disregards them - considering that it is "protein-stuff" from a system different from the one which an immune system is supposed to protect. The second: that they pass the digestive attacks fully functional. Some materials do, but very few retain all details in their structure while passing the stomach-purge. (cf enterosolvent coatings, etc.) I assign the passage through intestinal selections both to their size and built, so close to the 'partially' (more or less) digested other food stuf. Then they get to the sites where the adversive effect can be exercised - and do it. (I wonder if there were synthetic mad-cow prions prepared and inserted into experimental animals? There are so many chances - even in simple protein chemistry - that it may fail).
 
Does it really pass genetically? has it been excluded that e.g. the infected milk transferred the infection into the calves from infected mothers? If genetically, which way: through feeding channels into the fetus, or by sperm-egg content of parents, to begin with?
 
My reply to the problem is unfortunate: in spite of the importance of the problem and the lots of talks about it, we have no "real" knowledge about these (an many other) proceedings in the real complexity called globalizing: life. We know precious little about viruses and even less about prions, we still assign a considerable portion of the genetic material "garbage" and have no idea how mental processes (or even a disposition for a selective outcome) can be transferred "genetically" (memes?)  - if...
 
We cannot perfom 15 experiments in the lab, feed 500 lines of statistics into computers and solve the ignorance about the 'life' process (whatever it is). There are things beyond that selective and limited model we (science) carry about it and science is jealous to exclude every facet so far not included.
They really behave like a mad cow.
 
Happy New Year
 
John Mikes
 
 
 
----- Original Message -----
To: ***
Sent: Saturday, December 27, 2003 8:57 AM
Subject: Prion research and mad cow disease

Hi Folks,
 
The recent news stories of "mad cow disease" being found in American beef led me to do some research on this subject. I followed the suject into the territory of infectious agents and found that there are several sub-viral pathogenic agents that are only partly understood at present. Prions are one of those and a prion is believed to be the cause of "mad cow disease".
 
Does anyone on the list have further insight into these infectious agents? One site I studied contended that infection is both genetically passed and orally (ingestion) passed, which is an astounding assertion. If true, it's scary as hell. Prions are even more primitive than viruses, having only RNA and affecting the cell's manufacture of a single protein that is found in the cell membranes of normal cells, but the prion changes the shape of it. In my research, what I think I'm seeing is that this agent infects essentially A RELATIONSHIP in the cell, rather than the cell itself. It has a negative feedback effect on the production of the normal protein, causing more and more of the prion protein to be created in a cumulative way.
 
One piece of information I have yet to find is exactly how to protect one's self from infection. These prions can survive autoclave processing! They are not killed by normal antiviral (enzyme interfering) measures. They can survive a stomach environment and enter a host by passing through the intestinal wall with digested proteins-- and are ignored by the immune system! There appears to be a genetic predisposition to infection as well as a genetic immunity, depending on one's genes for creating this cell wall protein.
 
Any thoughts?
 
Judith